Extended Data Figure 1. Tomographic map of the human NPC.

(a) Orthoslices through the nucleocytoplasmic axis, CR, IR, and NR of a tomographic structure of the human NPC obtained using a direct electron detector (this study) compared to a structure obtained with a conventional detector using a similar experimental workflow (emd_2444). In both cases, the CR, IR and NR were aligned independently. The arrowhead indicates a transmembrane domain that is resolved in the IR. (b) Fourier shell correlation curves of the CR, IR and NR regions. (c) Histogram corresponding to the color-coded local resolution map shown in (d) that was calculated using ResMap ²⁵. (d) A single segment of the NR ring is shown (in all other figures the segments are shown jointly with their anterior and/or posterior asymmetric unit) at two different isosurface thresholds. The redundant density of the outer Nup43 beta propeller (horizontal arrowhead) and the Nup133 middle domain (vertical arrowhead) of two neighboring asymmetric units are indicated for orientation. The reduced resolution at the edges is due to the border of the mask used during alignment and averaging that covered about 1.5 times the asymmetric unit.