RT-qPCR analysis of NF-treated seedlings.
(A) Six-day-old FC1-OX line 250-1 seedlings were treated with 0.5 mM GAB and 1 mM ALA for 24 hr as indicated.
(B and C) NF-treated seedlings were grown in the presence of the indicated concentrations (B) or 1 mM ALA (C) for 6 days.
(D) Seven-day-old NF-treated seedlings of various genotypes as indicated below the axis. gun5 was additionally treated with 1 mM DP for 24 hr as indicated.
Data shown are the mean ± SEM of triplicate reactions of a representative experiment. See also Figure S3.