(A) Constitutively expressed FC1-YFP-HA and FC2-CFP-HA and natively expressed FC1-YFP and FC2-CFP colocalize to the same undeveloped plastids. Florescence was imaged by confocal microscopy; representative images are shown. Scale bars represent 30 µm.
(B) RT-qPCR analysis of steady-state mRNA transcript levels of 3-day-old NF-treated seedlings. Data shown are the mean ± SEM of triplicate reactions of a representative experiment.
(C) Western blot analysis of 7-day-old seedlings expressing FC1-YFP or FC2-YFP with their respective native promoters. See also Figure S4.