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. 2015 Oct 6;24(4):812–822. doi: 10.1038/mt.2015.154

Figure 1.

Figure 1

Derivation of X-linked chronic granulomatous disease (X-CGD) induced pluripotent stem cells (iPSCs) from plucked hair keratinocytes. (a) Diagram of the lentiviral reprogramming vector (LV-OKSM-Tomato). (b) Representative images of hair outgrowth, keratinocytes, and a derived iPSC colony (bars: left panel = 200 µm; middle and right panels = 100 µm). (c) Southern blot hybridized with the probe indicated in a showing lentiviral integrations in X-CGD iPSCs clones. Clone S22 carries three integrations and clone J14 two integrations. (d) The X-CGD iPSCs express endogenous pluripotency genes in similar levels to hESC line H7.S6. Data represent mean and SD of samples collected at day 100 and at day 125 and analyzed in triplicates. (e) X-CGD iPSCs express the pluripotency markers OCT4, NANOG, SSEA-3, SSEA-4, TRA-1–60, and TRA-1–81 and are negative for SSEA-1. Counterstaining with Hoechst 33342 and overlay is shown (bar = 100 µm). (f) X-CGD iPSCs gave rise to all three germ layers in teratoma formation assays. hESC, human embryonic stem cell.