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. 2016 Jan 5;24(4):736–745. doi: 10.1038/mt.2015.217

Figure 3.

Figure 3

Chemical inducer of dimerization (CID) response profiles of unique vector integration sites (VIS). For each gamma retroviral VIS, we estimate VIS copy number by tallying only sequencing read counts found within +100 bp of VIS, normalized by overall quantitative PCR transgene copy number per peripheral blood mononuclear cell sample. Time-scale plot of normalized VIS copy number for (a) Pt. 1, (b) Pt. 2, (c) Pt. 4, (d) Pt. 5. VIS present only before CID (pre-CID) were defined as “Eliminated” while VIS present at the last time point (i.e., late-CID) were considered “Persisted,” remaining VIS were termed “Short-term.” We also considered whether copy number change pre- versus post-CID infusion was either negative or positive (“Sensitive” versus “Insensitive”) to CID induction, respectively. Using these criteria, we segregated recovered VIS into four broad CID profiles (“Sensitive/Eliminated,” “Insensitive/Short-term,” “Sensitive/Persisted,” and “Insensitive/Persisted”).