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Measurement of the phase transition temperature by 31P NMR. The pH-sensitive cationic lipid was incorporated into a lipid dispersion with the neutral lipid POPC and the negatively charged lipid DSPS at a molar ratio of 1:1:1. Arrows indicate the signal of an inverted hexagonal phase. (a–c) YSK05. (d) YSK13-C2. (e) YSK13-C3. (f) YSK13-C4. DSPS, 1,2-distearoyl-sn-glycero-3-phosphatidylserine; NMR, nuclear magnetic resonance; POPC, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine.
