Impact of size on gene silencing in hepatocytes and in LSECs. (a) Mice were injected with siFVII formulated in YSK13-C3-MENDs with various size at 0.1 mg/kg and plasma FVII activities were measured in 24 hours. (b) Mice were injected with siCD31 formulated in YSK15-C4-MENDs with various size at 0.1 mg/kg and CD31 mRNA expression on LSECs were quantified in 24 hours. Data are represented as the mean ± SD (n = 3). *P < 0.05, **P < 0.01 (by one-way nrANOVA, followed by SNK test). ANOVA, analysis of variance; FVII, factor VII; LSEC, liver sinusoidal endothelial cell; MEND, multifunctional envelope-type nanodevice; nrANOVA, non-repeated analysis of variance; PBS, phosphate-buffered saline; SNK, Student-Newman-Keuls.