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. 2016 Jan 26;24(4):746–758. doi: 10.1038/mt.2015.232

Figure 2.

Figure 2

Formation of stable complexes between the novel vectors and siRNA. (a) siRNA was incubated for 45 minutes with different molar ratios of C2-9r or RI-C2-9r vectors. The mobility of free or vector-complexed siRNA was analyzed by agarose-gel electrophoresis. (b) Naked and vector-complexed siRNA were incubated in 50% mouse serum at 37 °C and aliquots taken at indicated time points were digested with proteinase K, electrophoresed on 15% urea-polyacrylamide gels and visualized by ethidium bromide staining. Free siRNA in water was included as control. The position of intact, uncomplexed siRNA is indicated. Diameter size (c) and zeta potential (d) of C2-9r/siRNA and RI-C2-9r/siRNA complexes are shown at 5, 15, 30, 45, and 60 min after complexing. Error bars represent standard error of mean of three independent samples. (e) Representative transmission electron microscope images of C2-9r, RI-C2-9r, siRNA, C2-9r/siRNA, and RI-C2-9r/siRNA after 45 minutes of complexing are shown. Arrows indicate single, magnified C2-9r/siRNA and RI-C2-9r/siRNA complexes. Bar = 500 nm.