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. 2016 Jan 26;24(4):746–758. doi: 10.1038/mt.2015.232

Figure 3.

Figure 3

Nonviral vectors mediate siRNA delivery and gene knockdown in vitro. (a) M17 cells were treated with rhodamine-siRNA alone or complexed with vectors and the intracellular localization of siRNA was confirmed by florescence microscopy after 24, 48, and 72 hours. Images of rhodamine-siRNA (red) positive cells are shown with the nuclei stained by DAPI (blue). Bar = 100 μm. (b) M17 cells stably expressing wild-type α-syn were transfected with siRNA using a commercial transfection agent or with vector complexed-SNCA siRNA and α-syn expression levels were evaluated 48 and 72 hours after transfection by western blotting. (c) α-Syn expression was normalized to beta-actin and the percentage of expression in transfected cells was plotted relative to untransfected cells. (d) M17 cells stably expressing wild-type α-syn were transfected with SNCA siRNA either by complexing with vectors or using a commercial transfection agent. 72 hours posttransfection, the cells were exposed to 2.5-mM MPP+ for 6 hours after which cell viability was quantified using MTT assay (***P < 0.0001, one-way ANOVA).