Figure 5.

Transgene expression could be upregulated by reactivation in vitro and makes the cells more sensitive to the dimerizer. (a) The expression of CD19 significantly increased (left panel) after ex vivo activation by OKT3/anti-CD28 antibody and CD3 (right panel), black line indicates the expression in peripheral blood and blue line indicates the expression in T cells reactivated by OKT3/anti-CD28. (b) In vitro drug sensitivity in CD3⁺CD19⁺ T cells with or without OKT3/anti-CD28 reactivation and (c) in vitro drug sensitivity in CD3⁺CD19⁻ populations. The percentage indicates the live cells in the population evaluated by 7AAD⁻/AnnV⁻. Representative example of three experiments is shown. 7-AAD, 7-amino-actinomycin; AnnV, Annexin V.