Figure 6.

Mutations detected in DNA after the third dimerizer treatment. iC9 transgene was amplified from the DNA extracted from peripheral blood mononuclear cells collected after the third administration of AP1903; multiple individual clones derived from the subcloned polymerase chain reaction products were sequenced. Nonsense mutations that generated a premature stop codon in the transgene at amino acid 61 were detected in the 2 out of 10 DNA samples and 1 out of 10 sample obtained 30 minutes and 4 days post #3 treatment, respectively (indicated in the red box). The mutations generated a stop codon at amino acid 61 instead of tryptophan (W61), (a) GG→AA (186–187), (b) G185A, and (c) No mutation.