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. 2016 May 31;11(5):e0156289. doi: 10.1371/journal.pone.0156289

Fig 10. Illustration summary describes the effect of dextran sodium sulfate (DSS) on the reference genes expression (RGE) stability in the gastrointestinal mucosa and their impact when used to quantify mRNA level of target genes using RT-qPCR.

Fig 10

Moreover, the immune cellular composition can greatly vary and can contribute to the variation of expression of a given gene. The least stable genes are the suboptimal genes that showed high variability (Gapdh, Actb, β2m, Trfc), while the most stable genes are the optimal genes that exhibited high stability (Tbp, Eef2).