Table 2. Summary of the diffraction characterization of Atk1 crystals grown by hanging drop vapour diffusion and the temperature-controlled dialysis method.
| Crystallization method | Hanging drop | Hanging drop | Hanging drop | Dialysis | Dialysis | Dialysis | Dialysis | Dialysis | Dialysis |
| PEG % | 25 | 25 | 25 | 21 | 20 | 19 | 19 | 18 | 18 |
| Largest dimension (µm) | 100 | 200 | 300 | 150 | 100 | 250 | 400 | 200 | 400 |
| Resolution† (Å) | 50–1.85 (1.96–1.85) | 50–1.79 (1.90–1.79) | 50–1.87 (1.99–1.87) | 50–1.61 (1.71–1.61) | 50–1.67 (1.77–1.67) | 50–1.55 (1.64–1.55) | 50–1.34 (1.42–1.34) | 50–1.41 (1.49–1.41) | 50–1.22 (1.29–1.22) |
| Mosaicity (°) | 0.354 | 0.397 | 0.578 | 0.270 | 0.223 | 0.187 | 0.259 | 0.205 | 0.259 |
| I/σ(I)† | 14.54 (2.03) | 13.64 (2.07) | 9.29 (2.17) | 13.74 (2.08) | 16.60 (2.01) | 14.61 (2.01) | 13.77 (2.06) | 13.41 (2.08) | 13.12 (2.07) |
| CC(1/2)‡ | 99.9 (71.4) | 99.8 (78.8) | 99.6 (91.0) | 99.8 (74.0) | 99.9 (69.7) | 99.9 (69.0) | 99.8 (74.3) | 99.8 (74.3) | 99.8 (79.6) |
†
Highest resolution shell in parentheses.
‡
Karplus & Diederichs (2012 ▸).