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. 2016 May 31;11(5):e0156727. doi: 10.1371/journal.pone.0156727

Table 1. Primers used in this study to detect pathogens in deer keds (Lipoptena fortisetosa) collected from Korean water deer.

Species Target gene Name of primer Primer sequence (5’−3’) Expected size (bp) Reference
L. fortisetosa cox-1 CI-J-1632 TGA YCA AAT TTA YAA Y 730 Modified from [37]
CI-N-2329 ACT GTA AAT ATR TGA TGA GCT CA
CI-J-1718 GGA TTT GGW AAT TGA YTA RTW CC 519
CI-N-2191 GGT AAA ATT AAA ATA TAA ACT TC
A. phagocytophilum 16S rRNA EE1 TCC TGG CTC AGA ACG AAC GCT GGC GGC 1433 [38]
EE2 AGT CAC TGA CCC AAC CTT AAA TGG CTG
EE3 GTC GAA CGG ATT ATT CTT TAT AGC TTG C 928
EE4 CCC TTC CGT TAA GAA GGA TCT AAT CTC C
Borrelia spp. 5S–23S rRNA Bb23S3 CGA CCT TCT TCG CCT TAA AGC 412 [39]
Bb23Sa TAA GCT GAC TAA TAC TAA TTA CCC
Bb23S3nF CTG CGA GTT CGC GGG AGA 226–266
Bb23SanR TCC TAG GCA TTC ACC ATA
Coxiella spp. 16S rRNA Cox16SF1 CGT AGG AAT CTA CCT TRT AGW GG 1321–1416 [23]
Cox16SR2 GCC TAC CCG CTT CTG GTA CAA TT
Cox16SR1 ACT YYC CAA CAG CTA GTT CTC A 719–813
Bartonella spp. ITS-1 QHVE-OF TTC AGA TGA TGA TCC CAA GC 736 [15]
QHVE-OR AAC ATG TCT GAA TAT ATC TTC
QHVE-IF CCG GAG GGC TTG TAG CTC AG 484
QHVE-IR CAC AAT TTC AAT AGA AC
Hepatozoon spp. 18S rRNA HepFc ATA CAT GWG CAM AWT CTC AAC 680 [40]
HepRc TTA TWA TTC CAT GCT GCA G
T. ovis 18S rRNA To18F CGA ATC GCG TCT TCG GAT GCG 418 In this study
To18R GCC ACA ATG CAA AGA CTC G
T. luwenshuni 18S rRNA Tlw18F GAA TCG CAG CTT TTG CGG CG 1061 In this study
Tlw18R ATA CCC GCA TCC TAT TTA GCA GG