Table 1. Primer sequences and annealing temperatures used in real time PCR to detect cytokine and cytokine receptor expression in the spleens of dogs with visceral leishmaniasis.

Gene	Forward primer (5’-3’)	Reverse primer (5’-3’)	Annealing temperature (°C)
18S	CACGGCCGGTACAGTGAAAC	CCCGTCGGCATGTATTAGCT	60°
APRIL	CTTTCACCATGGGTCAGGTGGTGTC	GACACTCAGAATATCGCCCTGGTGC	60°
BAFF	TCTTTGGGGATGAACTGAGC	CAGAAGCTTCAATGCACCAA	55°
IL6	GGCTACTGCTTTCCCTACCC	TTTTCTGCCAGTGCCTCTTT	55°
CXCR4	CAGTTGAGGCTGTGGCAAAC	GAGAGCAGGTATCCAGACGC	55°
CXCL12	TCTTCGAGAGCCACATTGCC	GGGTCAATGCACACCTGTCTG	60°