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. 2016 May 31;11(5):e0156054. doi: 10.1371/journal.pone.0156054

Fig 2. Intracellular ROS-production in HL-1 cells is not directly affected by mitoNEET.

Fig 2

HL-1 cells were transfected with silencing RNA (siRNA) directed against mitoNEET and non-specific-siRNA as control and subjected to 3h hypoxia followed by 1h of reoxygenation (H/R). Using the fluorescent probe DCF-DA intracellular ROS-production was analyzed in a microplate reader. After 3h hypoxia intracellular ROS is 2.6 fold and after 60 min of reoxygenation even 5.4 fold increased compared to normoxic control cells (n = 12). Addition of antioxidative superoxide scavenger Tiron (10 mM; n = 6) and GSH-MEE (2 mM; n = 9) reduced the amount of ROS significantly. MitoNEET-KD doesn’t show any effect on the amount of intracellular ROS measured within the different conditions.