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. 2016 May 31;11(5):e0156054. doi: 10.1371/journal.pone.0156054

Fig 5. 2-AAPA decreases H/R induced apoptosis in mitoNEET-KD cells and increases mitoNEET-protein despite KD.

Fig 5

(A) Western Blot analysis of activated caspase-3 revealed reduced apoptosis in 2-AAPA treated mitoNEET-KD cells to the same level seen in 2-AAPA treated HL-1 cells after H/R (n = 6). (B) siRNA transfection decreased mitoNEET-mRNA efficiently in the presence and absence of chemical GSR-inhibitor 2-AAPA (10 μM, n = 5) determined by real-time RT-PCR. (C) MitoNEET-protein of mitoNEET-KD-cells was significantly higher after addition of 2-AAPA compared to mitoNEET-KD-cells without 2-AAPA treatment (n = 5) measured after H/R. Representative Western Blots are shown (A, C) and data are expressed as % of H/R control. Real-time RT-PCR data are expressed as 2-ΔΔCT.