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. Author manuscript; available in PMC: 2016 May 31.
Published in final edited form as: Cell Rep. 2016 Jan 21;14(4):872–884. doi: 10.1016/j.celrep.2015.12.084

Figure 8. Overexpression of Mps1 in RCC Increases Hsp90 Binding to GB.

Figure 8

(A–F) ccRCC (A), papillary type I (B and C), papillary type II (D), oncocytoma (E), and AML (F) tissues were cultured in medium with 10 μM NMS-P715 (Mps1 inhibitor) or without drug for 24 hr. After protein lysate preparation, Hsp90 binding to GB was assessed by biotinylated-GB and immunoblotting.

(G) Hsp90 in samples (A)–(F) was detected by immunoblotting using anti-Hsp90-antibody.

(H) Overexpression and hyperactivity of Mps1 in kidney tumor compared to the adjacent normal tissue confer cancer cell selectivity and sensitivity to Hsp90 inhibitor GB.