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. Author manuscript; available in PMC: 2017 Feb 1.
Published in final edited form as: J Cell Biochem. 2015 Sep 1;117(2):533–541. doi: 10.1002/jcb.25306

Fig. 3.

Fig. 3

FOXD3 is not necessary for PAX3 expression in melanoma cells. (A) Schematics of the FOXD3 protein (top) and the portion of FOXD3 used in the FOXD3 dominant negative construct (FOXD3-DN, bottom). The C-terminal half of the FOXD3 protein starting from the nuclear localization signal (NLS) through the end of the protein was cloned into pcDNA3 to create FOXD3-DN. (B) Inhibition of FOXD3 activity does not alter PAX3 protein levels in melanoma levels. Western blots were performed on A375 and mel-624 cells transfected with the FOXD3-DN construct or pcDNA3 (as a negative control) and probed for FOXD3, PAX3, and vinculin (loading control). (C) Densitometric measurements of PAX3 levels in western blots.