Figure 2. Repeated injection of the PEGylated, but not the non-PEGylated, htCBS shows buildup of CBS activity in vivo.

C57BL/6J mice were injected at 0, 24, 48 hours (indicated by arrows) with 5 mg/kg BW of the non-PEGylated htCBS (n = 5) or with GL4-400MA PEGylated htCBS (n = 5) and were bled at the indicated time points. Data are presented as the mean ± SEM and were compared using an unpaired Student’s t test. **P ≤ 0.01 and ***P ≤ 0.001.