Figure 5. ADAMTS3 is required for lymphatic development in mouse embryos.

(A) Loss of ADAMTS3 resulted in severe cutaneous edema at E14.5. Arrow indicates translucent space between the skin and body, indicative of edema. (B) Loss of ADAMTS3 resulted in a complete lack of PROX1⁺LYVE1⁺ LECs in the E14.5 embryo. Note the presence of smaller lymphatic vessels in the skin and larger lymphatic vessels adjacent to the dorsal aorta and cardinal vein in the WT littermate control embryo. (C) ADAMTS3 was not required for blood vessel development. Blood vessels from an ADAMTS3-deficient and control embryo were identified using anti-PECAM and anti-FLK1 immunostaining. cv, cardinal vein; da, dorsal aorta. Data shown are representative of 3 separate experiments. Scale bars: 50 μm.