Figure 1. GF mice are protected against the loss of trabecular bone induced by sex steroid deficiency.
Assessment of femoral bone structure by in vitro μCT in Conv.R mice, GF mice, and Col.GF mice following either leuprolide (375 μg/month) or vehicle control treatment for 10 weeks. (A) Images of representative 3-dimensional μCT reconstructions of examined femurs from each group. (B) BV/TV. (C) Ct.V. (D) Ct.Th. (E) Tb.N. (F) Tb.Sp. (G) Tb.Th. n = 10 mice per group in all panels. Data are expressed as mean ± SEM. All data were normally distributed according to the Shapiro-Wilk normality test and analyzed by 2-way ANOVA and post hoc tests applying the Bonferroni correction for multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared with the indicated groups.