Figure 7. SHP2 activity is not involved in autoantibody production.

(A) Serum IgG and (B) anti-dsDNA IgG were measured in 18-week-old WT, MpJ, and MRL/lpr female mice treated for 6 weeks with vehicle or 11a-1 (7.5 mg/kg/d). n = 7–8 mice/group. Representative flow cytometry and corresponding quantification of (C) extrafollicular T helper cells (Tefh) (TCRβ⁺CD4⁺PSGL-1^–CD62L^–) and (D) plasmoblasts (TCRβ^–CD44^hiB220⁺CD138⁺) and plasma cells (TCRβ^–CD44^hiB220^–CD138⁺) isolated from spleens of 18-week-old WT, MpJ, and MRL/lpr female mice treated for 6 weeks with vehicle or 11a-1 (7.5 mg/kg/d). n = 3–5 mice/group. *P < 0.01; ^#P < 0.05, 2-way ANOVA with Holm-Sidak post-test when ANOVA was significant.