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. 2016 May 16;126(6):2077–2092. doi: 10.1172/JCI87037

Figure 8. SHP2 also modulates proliferation of T cells and expression of IFN-γ and IL-17A/F in human lupus.

Figure 8

Serum levels of (A) IL-17A/F and (B) IFN-γ were measured from either normal or lupus patients with SLEDAI scores higher than 7. n = 24–25/group. (C) Representative images of cultured normal or lupus patient T cells, isolated from PBMCs, following 48 hours of culture in the presence of vehicle (DMSO) or 11a-1 (10 μg/ml) and in the absence or presence of activating antibodies anti-CD3 and anti-CD28. Scale bar: 500 μm. T cells isolated from normal or lupus patients were assessed for (D) proliferation and (E) viability (n = 3 independent experiments) after 48 hours of culture. In addition, T cells isolated from normal or lups patients were measured for levels of (F) IL-17A/F or (G) IFN-γ following 120 hours of culture. n = 12–13/ group. *P < 0.05, 1-way or 2-way ANOVA with Holm-Sidak post-test when ANOVA was significant.