Video 2. A cell with a mispositioned spindle that inappropriately exits mitosis in the mother cell compartment.
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DOI: 10.7554/eLife.14036.006
osTIR1 DYN-AID kar9Δ cells expressing GFP-labeled α-tubulin (A35707) were grown and imaged as described in Figure 1E-I. Depicted is a cell that mis-positions its spindle and then inappropriately exits mitosis in the mother cell. Time in minutes is show in the upper left-hand corner of the time-lapse image. A merged image of the DIC and GFP channels is shown on the left. The GFP channel alone is shown on the right. The DIC image was adjusted to maximize contrast.