Video 3. A cell with a mispositioned spindle that inappropriately exits mitosis but does not complete spindle disassembly or cytokinesis.
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DOI: 10.7554/eLife.14036.007
osTIR1 DYN-AID kar9Δ cells expressing GFP-labeled α-tubulin (A35707) were grown and imaged as described in Figure 1E-I. Depicted is a cell that mispositions its spindle and then inappropriately exits mitosis in the mother cell but does not complete cytokinesis or spindle disassembly. Time in minutes is show in the upper left-hand corner of the time-lapse image. A merged image of the DIC and GFP channels is shown on the left. The GFP channel alone is shown on the right. The DIC image was adjusted to maximize contrast.