Video 4. A cell with a mispositioned spindle that lacks cMT-bud neck interactions but arrests in anaphase.
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DOI: 10.7554/eLife.14036.008
osTIR1 DYN-AID kar9Δ cells expressing GFP-labeled α-tubulin (A35707) were grown and imaged as described in Figure 1E-I. Depicted is a cell that arrests in late anaphase with a mispositioned spindle. This cell lacks cMTs in the bud neck for a substantial time period in anaphase. Time in minutes is show in the upper left-hand corner of the time- lapse image. A merged image of the DIC and GFP channels is shown on the left. The GFP channel alone is shown on the right. The DIC image was adjusted to maximize contrast.