Figure 7. IFIT1, but not IFIT1B, inhibits replication of VSV, a cap1-containing virus.
(A) Cell lines were infected with vesicular stomatitis virus (VSV) encoding a GFP protein at an MOI of 0.01. After 12 hr, cells were visualized for expression of the virally-encoded GFP. (B) Infections were performed as in part A, but at the indicated timepoint, virus was harvested and titered. Error bars represent standard deviation of three biological replicates. (C) Infections were performed as in part A, harvested after 12 hr, and titered. Error bars represent standard deviation of three biological replicates. (D) In contrast to IFIT1B, IFIT1 inhibits VSV growth arrest regardless of the fact that cap1-methyltransferase is present.