(
A) Principal component (PC) analysis of methylation frequencies at DMPs in each generation, with full information across all samples of that generation. Numbers in brackets indicate the percentage of variation explained by the respective PC. (
B) Complete linkage clustering of pairwise correlation of DMP methylation frequencies, separated by generation. (
C) Fraction of hyper- and hypo-methylated DMPs after salt-treatment of P0 samples. (
D and
E) Analyses based on data published by
Jiang et al. (2014). G1: generation 1. G10: plants after 10 generations of either control of NaCl treatment. (
D) Reanalysis of published hyperosmotic stress data (
Jiang et al., 2014). PC analysis of methylation frequencies at DMPs (left panel). Bi-hierarchical clustering of pairwise correlation of methylation frequency at DMPs identified in all pairwise comparisons, with full information across all samples (right panel). (
E) Reanalysis of previously published hyperosmotic stress data (
Jiang et al., 2014). Complete linkage clustering based on DMR methylation frequencies, divided by sequence context. The methylation frequency of each DMR per sample was calculated as the average methylation frequency of cytosines in that DMR. Only DMRs covered in each sample were considered. G1, untreated generation 1; G10, generation 10; C, control; S, salt-treated.