TABLE 1.
Cell cycle phase distribution of CGN-exposed and unexposed NCM460 cells1
| 2 d
|
4 d
|
6 d
|
8 d
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| CGN | Control | CGN | Control | CGN | Control | CGN | Control | ||
| Attached cells | % | ||||||||
| G0-G1 | 86.4 ± 0.3 | 86.0 ± 0.8 | 83.4 ± 1.0 | 81.0 ± 0.4 | 69.8 ± 0.1 | 85.7 ± 0.2 | 70.4 ± 1.0 | 86.5 ± 0.5 | |
| S | 5.5 ± 0.4 | 5.4 ± 0.4 | 7.9 ± 0.5 | 9.5 ± 0.01 | 12.1 ± 0.2 | 5.4 ± 0.3 | 11.2 ± 0.2 | 5.7 ± 0.5 | |
| G2-M | 4.4 ± 0.04 | 4.2 ± 0.1 | 5.9 ± 0.3 | 6.7 ± 0.4 | 8.3 ± 0.06 | 4.9 ± 0.4 | 8.4 ± 0.2 | 3.8 ± 0.4 | |
| Sub-G0 | 2.1 ± 0.7 | 3.0 ± 0.2 | 1.6 ± 0.8 | 1.2 ± 0.7 | 6.7 ± 0.5 | 2.6 ± 0.1 | 6.9 ± 1.3 | 2.8 ± 0.5 | |
| Detached cells | |||||||||
| G0-G1 | 62.8 ± 2.3 | 56.3 ± 1.7 | 59.8 ± 2.7 | 58.8 ± 1.0 | |||||
| S | 8.3 ± 0.01 | 10.8 ± 0.5 | 9.2 ± 1.0 | 10.2 ± 0.4 | |||||
| G2-M | 9.2 ± 0.6 | 8.1 ± 0.9 | 9.0 ± 0.6 | 7.0 ± 1.0 | |||||
| Sub-G0 | 11.9 ± 0.5 | 18.5 ± 0.6 | 15.3 ± 2.8 | 18.4 ± 1.5 | |||||
1
Values are means ± SD of 2 independent measurements, each of which measured >10,000 cells in the attached cells, and 5000–10,000 in the detached cells. Differences between CGN-exposed and control cells were significant at d 6 and 8 for the G0-G1, S, and G2-M cell cycle phases, P < 0.001; 1-way ANOVA with Tukey-Kramer post test, and for the sub-G0 phase, P < 0.01. In the detached cells, only the difference in CGN-treated vs. control cells in S-phase on d 6 achieved significance, P < 0.05.