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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: J Ethnopharmacol. 2016 Apr 22;187:249–258. doi: 10.1016/j.jep.2016.04.034

Figure 4. GM fails to block glutamate-induced GHS depletion and does not directly scavenge intracellular ROS.

Figure 4

(A) GSH levels were detected at 9 h after exposure to glutamate (5 mM) and GM (25 μM) (n=5). (B) HT-22 cells were exposed to H2O2 (50 μM) and GM (25 μM) for 20 min. ROS levels were detected by H2DCF and fluorescence was measured by plate reader (n=8). All experiments were repeated three times and the results indicate the mean ± SEM. ***P < 0.001 compared with control (one-way ANOVA, Tukey’s test).