Figure 6. GM enhances glycolytic activity.
After a 10 h exposure to glutamate (5 mM) and GM (25 μM) (A) or a 24 h exposure to GM (5–50 μM) (B), ECAR was recorded by a Seahorse XFe96 flux analyzer (n=8). ECAR recording at baseline and subsequent exposure toglucose (10 mM), oligomycin(5 μM) and 2-DG (100 mM). Glycolysis, glycolytic reserve, glycolytic reserve capacity and non-glucose-derived ECAR were calculated. All experiments were repeated three times and the results indicate the mean ± SEM. *P < 0.05, **P < 0.01, *** P < 0.001 compared with control. #P < 0.05, ### P < 0.001 compared with glutamate-treated cells. (one-way ANOVA, Tukey’s test).