Table 2.
Global stability parameters from GdnHCl denaturation of Cytc variants at 25 °C and pH 7.
| Variant | ΔGu°(H2O) | m | Cm |
|---|---|---|---|
| Human | 8.9 ± 0.2 | 3.51 ± 0.06 | 2.54 ± 0.01 |
| K8R | 9.0 ± 0.1 | 3.48 ± 0.04 | 2.58 ± 0.04 |
| K8R/P44S | 8.8 ± 0.2 | 3.52 ± 0.11 | 2.51 ± 0.02 |
| K8R/P44S/Y46F | 8.7 ± 0.2 | 3.44 ± 0.17 | 2.52 ± 0.06 |
| K8R/P44S/Y46F/S47T | 9.0 ± 0.1 | 3.66 ± 0.03 | 2.45 ± 0.01 |
| K8R/P44S/Y46F/S47T/A50E (Spider Monkey) |
8.7 ± 0.4 | 3.49 ± 0.19 | 2.48 ± 0.02 |
| Equinea | 7.27 | 3.01 | 2.42 |
| Bovinea | 8.38 | 3.19 | 2.62 |
| Yeastb | 5.77 ± 0.4 | 5.11 ± 0.36 | 1.13 ± 0.02 |