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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Inorg Biochem. 2015 Dec 31;158:62–69. doi: 10.1016/j.jinorgbio.2015.12.025

Table 4.

Parameters for the alkaline conformational transition of SM Cytc at 23 ± 1 °C as a function of solution ionic strength.

Ionic Strengtha Spider Monkey
(E. Coli-expressed)		 Spider Monkey
(Natural Source)b
pKapp n pKapp
10 mMc 9.21 ± 0.03 0.79 ± 0.04 8.70 ± 0.05
50 mM 9.15 ± 0.05 0.78 ± 0.01 _
250 mM 9.22 ± 0.02 0.89 ± 0.05 9.30 ± 0.05
a

Ionic strength adjusted with NaCl in the presence of 10 mM Tris acetate

b

Values from ref. [15]. Values of n not reported individually, a range of 0.8 to 1.1 was indicated. 10 mM value was obtained in the presence of Tris acetate. Ionic strength for 250 mM was adjusted with sodium acetate.

c

10 mM Tris acetate, no NaCl added.