Fig. 10.

Alleviation of dusk FLOWERING LOCUS T (FT) transcript suppression over 2 wk may be caused by the change in FLOWERING LOCUS M (FLM)-β and FLM-δ transcripts in Arabidopsis thaliana (Columbia-0 (Col-0)). (a) SVP transcript accumulation in wild-type plants grown in LD22/22°C (long days with day: night temperatures = 22°C: 22°C) and LD22/12°C conditions in wild type for 14 d harvested at zeitgeber time (ZT) 0, ZT8, and ZT16 starting on day 1 of treatment. Accumulation patterns (b) and quantification (c) of SVP-6HA protein in SVP:SVP-6HA plants grown in LD22/22°C and LD22/12°C conditions over the 2 wk, harvested every other day at ZT13 starting on day 1. HSP90 serves as a loading control. (d) FLM-β and (e) FLM-δ splice variant transcript accumulation patterns and (f) the ratio of FLM-β to FLM-δ in wild-type plants grown in LD22/22°C and LD22/12°C for 14 d harvested at ZT0, ZT8, and ZT16 starting on day 1 of treatment. The ratios (f) were calculated for each time point and then averaged over each day. In all cases plants were grown in short days (SD) for 1 wk before being transferred to treatments. Data represent means ± SEM derived from three biological replicates. (a, e, f) Asterisks (*) indicate P<0.05 and 95% CI of difference between pair did not contain zero.