Fig. 2.

Flowering and transcript accumulation patterns of CONSTANS (CO) and FLOWERING LOCUS T (FT) in different Arabidopsis thaliana ecotypes in response to day: night temperature fluctuations are similar to those in the Columbia-0 (Col-0) Arabidopsis thaliana accession. (a) Rosette leaf number of Col-0, Landsberg erecta (Ler), and Wassilewskija-2 (Ws-2) (top) and percent change in rosette leaf number between treatment means within a replicate (bottom) in LD22/22°C (long days with day: night temperatures = 22°C: 22°C) and LD22/12°C conditions. (Top) Asterisks (***) indicate significance between treatments (P<0.001), mean and 95% Confidence Interval (CI, 0.329, 0.232, 0.425), (0.313, 0.206, 0.419), and (0.290, 0.183, 0.397), respectively. (Bottom) Differences in percent change from that of Col-0 for Ler and Ws (P = 0.949 and 0.844, respectively), mean and 95% CI (−0.408, −1.908, 1.092) and (−0.549, −2.049, 0.951), respectively, (replicate = 5, n = 10). (b, c) CO transcript accumulation and (d, e) FT transcript accumulation in Ler and Ws in LD22/22°C and LD22/12°C conditions. Seedlings were grown under the same conditions used for the experiments shown in Fig. 1. Data represent means ± SEM derived from at least three biological replicates. (b–e) Asterisks (*) indicate P<0.05 and 95% CI of difference between pair did not contain zero. ZT, zeitgeber time.