Fig. 2A–D.

Luciferase assays were performed to assess the effect of the DNA-protein interactions on TPM2 promoter activity for rs2025126/TPM2 and rs2145925/TPM2. The TPM2 promoter construct incorporated 460-bps upstream of the transcriptional start site and was ligated into the pGL4.10 basic luciferase vector. The ancestral and alternate allele constructs were generated by ligating the electrophoretic mobility shift assay double-stranded oligonucleotides that contained (A) rs2025126 or (B) rs2145925 in front of the TPM2 promoter construct. (C) The ancestral allele of rs2025126 that creates a DNA-protein interation caused a decrease in promoter activity. (D) The alternate allele of rs2145925 creating the DNA-protein interaction causes an increase in promoter activity.