A. The SHH ligand patched 1 constitutively represses SMO, allowing the sequential phosphorylation of Gli by protein kinase A (PKA), glycogen synthase kinase 3 beta (GSK3B), and casein kinase (CK). Phosphorylation of the Gli family zinc finger proteins marks them for ubiquitination and limited proteosomal degradation. N-terminal fragments of Gli proteins translocate to the nucleus where they repress transcription.
B. Binding of SHH to patched 1 prevents its inhibition of SMO. This prevents phosphorylation and degradation of Gli proteins. Full-length Gli proteins translocate to the nucleus and promote transcription.