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. 2016 Jun 1;7:145. doi: 10.3389/fphar.2016.00145

FIGURE 1.

FIGURE 1

Effects of rhEndostatin on RANKL-induced osteoclast-like cell formation in mouse BMMs. (A) Mouse BMMs were cultured with vehicle (distilled water) or the indicated concentrations of rhEndostatin for 3 h, then stimulated with M-CSF (30 ng/mL) and RANKL (100 ng/mL) for 5 days. The osteoclasts were fixed and stained for TRAP staining. (B) TRAP-positive multi-nucleated cells with greater than three or more nuclei were counted as osteoclasts. (C) Effects of rhEndostatin on the cell viability of BMMs. BMMs were treated with the indicated concentrations of rhEndostatin for the indicated times. Cell viability was determined by the MTT method. ∗∗P < 0.01 compared with vehicle-treated control.