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. 2016 Jun 1;7:145. doi: 10.3389/fphar.2016.00145

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Copyright © 2016 Chen, Gao, Yuan and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of rhEndostatin on osteoclast-like cell formation treated for different time periods. (A) Schematic of the experimental design for the in vitro experiments. BMMs were cultured in the presence of M-CSF and RANKL for 5 days, and rhEndostatin (50 mM) was added to the BMM cultures at the indicated days. (B) The number of TRAP-positive multi-nucleated cells were counted. The data are presented as percentages relative to the vehicle-treated control group. ^∗∗P < 0.01 compared with the vehicle-treated control.