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. 2016 Jun 1;7:145. doi: 10.3389/fphar.2016.00145

FIGURE 5.

FIGURE 5

Effect of rhEndostatin on RANKL-induced activation of NF-κB in BMMs. (A) BMMs were transiently co-transfected with NF-κB luciferase reporter gene constructs, then pre-treated with vehicle or the rhEndostatin (12.5, 25, and 50 mM) for 3 h. RANKL was added and cells were incubated for an additional 12 h. The cells were harvested and the level of NF-κB activation was determined using the luciferase reporter assay system. ∗∗P < 0.01 compared with the vehicle-treated control (B) BMMs were pre-treated with vehicle or the rhEndostatin (50 mM) for 3 h, then stimulated with RANKL (100 ng/mL) for the indicated times. The cytosolic level of phosphorylated NF-κB IκBα and the nuclear phosphorylation of p65 levels were determined by western blot analysis. (C) Quantification of phosphorylated NF-κB IκBα and phosphorylation of p65 protein expression were normalized by total IκBα and p65, respectively. ∗∗P < 0.01 compared with the vehicle-treated control.