FIGURE 6.

Effect of rhEndostatin on RANKL-induced activation of MAPK in BMMs. (A) BMMs were pre-treated with vehicle or rhEndostatin (50 mM) for 3 h, then stimulated with RANKL (100 ng/mL) for the indicated times. Total protein was extracted and analyzed by western blot analysis using antibodies against phospho-ERK1/2, ERK1/2, phospho-JNK, JNK, phospho-p38, and p38. (B) Quantification of phosphorylated ERK1/2, p38, and JNK protein expression was normalized by total ERK1/2, p38, and JNK expression, respectively. ^∗∗P < 0.01 compared with the vehicle-treated control.