FIGURE 3.

Genetic complementation of the lip5 mutant in response to ABA. (A) Transcript levels of AtLIP5 in the Col-0 and lip5-s123 and lip5-s145 mutants determined by RT-PCR. (B) Representative phenotypes of seedlings at 7 days after the end of stratification. Twenty-four hours after stratification, germinated seeds were transferred from ABA-free medium to medium containing 1 or 2 μM ABA. (C) Root growth measurements. Seedling root length was measured at 7 days after the end of stratification. Relative root growth compared with that on ABA-free medium is indicated. Data show the mean ± SE of three replicates. At least 30 seedlings per genotype were measured in each replicate. T-test, with ^∗∗P < 0.01; t-test, with ^∗P < 0.05. In both (B,C) experiments were repeated at least two times with similar results.