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. 2016 Jun 1;10:147. doi: 10.3389/fncel.2016.00147

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Copyright © 2016 Guglielmotto, Reineri, Iannello, Ferrero, Vanzan, Miano, Ricci, Tamagno, De Bortoli and Cutrupi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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NGB mRNA and protein expression in SK-N-BE and NT-2 differentiated neuroblastoma cells. (A) Time-course of NGB mRNA after 10 nM 17β-estradiol (E2) treatment. Error bars are ± SEM of three independent biological replicates: *p-value < 0.05, **p-value < 0.01 and ***p-value < 0.001. The p-values were calculated with one-way analysis of variance (ANOVA) followed by Bonferroni post hoc test. (B) Time-course of NGB protein after 10 nM E2 treatment. In all cases diagram shows the mean normalized densitometry values.