Figure 5.
E2 induces estrogen receptor alpha (ERα) association on promoter and intron regions of differentiated SK-N-BE and NT-2 neuroblastoma cells. ChIP-qPCR analysis of ERα binding at the promoter, intronic enhancer (Site B) and negative control region, GAPDH, of differentiated SK-N-BE (A) and NT-2 (B) cells, treated with 10 nM E2 for 45 min and 1 h. Error bars are ± SEM of three independent biological replicates.