Table 1.
Primary and secondary antibodies applied for immunohistochemistry of retinal and optic nerve tissue.
| Primary antibodies |
Secondary antibodies |
||||||
|---|---|---|---|---|---|---|---|
| Antibody | Company | Tissue | Dilution | Antibody | Company | Tissue | Dilution |
| Brn-3a | Santa Cruz | Retina | 1:100 | Donkey anti-goat Alexa Fluor 488 | Dianova | Retina | 1:1000 |
| C1q | Quidel | Retina | 1:2500 | Donkey anti-goat IgG Cy3 | Abcam | Retina | 1:750 |
| C3 | Cedarlane | Retina | 1:500 | Goat anti-rabbit IgG Cy 3 | Linaris | Retina | 1:500 |
| Optic nerve | 1:500 | Optic nerve | 1:500 | ||||
| C5b-9 (MAC) | Biozol | Retina | 1:100 | Donkey anti-mouse Dy Light 488 | Dianova | Retina | 1:250 |
| Optic nerve | 1:100 | Goat anti-mouse Alexa Flour 488 | Invitrogen | Optic nerve | 1:500 | ||
| MASP2 | Biozol | Retina | 1:400 | Donkey anti-rabbit Alexa Fluor 555 | Invitrogen | Retina | 1:400 |
| Optic nerve | 1:100 | Optic nerve | 1:700 | ||||
| MBP | Millipore | Optic nerve | 1:100 | Goat anti-mouse Alexa Flour 488 | Invitrogen | Optic nerve | 1:500 |