Table 2.
Sequences of oligonucleotide pairs.
| Gene | Forward (F) and reverse (R) oligonucleotides | GenBank accession number | Amplicon size |
|---|---|---|---|
| β-actin-F β-actin-R |
cccgcgagtacaaccttct tcaagcggtacctactgc |
NM_031144.3 | 72 bp |
|
C1qa-F C1qa-R |
cgggtctcaaaggagagagag ctctgtacccccttagacc |
NM_001008515.1 | 88 bp |
|
C1qb-F C1qb-R |
gcactccagggataaaagga accactcaatcctctctttccc |
NM_019262.1 | 75 bp |
|
C3-F C3-R |
tcgaaatccctcccaagtc gtaacaggggaacttctagc |
NM_016994.2 | 60 bp |
|
C5-F C5-R |
tctcaggccaaagagagacc ttcgacgatttatgtttgtggca |
XM001079130.4 | 73 bp |
|
Cyclophilin-F Cyclophilin-R |
tgctggaccaaacacaaatg tcgtacaccagaaacccttc |
M19553.1 | 88 bp |
|
MASP2-F MASP2-R |
gctggaagatacactacacaagca gtggattaccagtgtaaagtgg |
NM_172043.1 | 76 bp |
The listed primer pairs were used in quantitative real-time PCR experiments, while β-actin and cyclophilin served as housekeeping genes. The predicted amplicon sizes are given. Abbreviations: F, forward; R, reverse; bp, base pair.