Table 1.
Binding affinities of all of the selected compounds to the recombinant AlinOBP11 protein.
| Ligand | CAS Number | AlinOBP11 | |
|---|---|---|---|
| IC50 (μM) | Ki (μM) | ||
| GENERAL ODORANTS | |||
| 2-Hexanol | 626-93-7 | u.d. | u.d. |
| Pentanol | 71-41-0 | u.d. | u.d. |
| Valeraldehyde | 110-62-3 | u.d. | u.d. |
| Hexanal | 66-25-1 | u.d. | u.d. |
| Heptanal | 111-71-7 | u.d. | u.d. |
| Octanal | 124-13-0 | u.d. | u.d. |
| Nonanal | 124-19-6 | u.d. | u.d. |
| 2-Hexanone | 591-78-6 | u.d. | u.d. |
| 2-Heptanone | 110-43-0 | u.d. | u.d. |
| 2-Octanone | 111-13-7 | u.d. | u.d. |
| 3-Hexanone | 589-38-8 | u.d. | u.d. |
| 6-Methyl-5-hepten-2-one | 110-93-0 | u.d. | u.d. |
| Amyl acetate | 628-637-7 | u.d. | u.d. |
| Nonyl acetate | 1143-13-5 | u.d. | u.d. |
| Undecane | 1120-21-4 | u.d. | u.d. |
| Indole | 120-72-9 | u.d. | u.d. |
| Benzaldehyde | 100-52-7 | u.d. | u.d. |
| 3,4-Dimethyl-benzaldehyde | 5973-71-7 | u.d. | u.d. |
| Acetophenone | 98-86-2 | u.d. | u.d. |
| Methyl salicylate | 119-36-8 | u.d. | u.d. |
| GREEN LEAF VOLATILES | |||
| 1-Hexanol | 111-27-3 | u.d. | u.d. |
| cis-3-Hexen-1-ol | 928-96-1 | u.d. | u.d. |
| trans-2-Hexenal | 6278-26-3 | u.d. | u.d. |
| cis-3-hexenyl acetate | 3681-71-8 | u.d. | u.d. |
| TERPENOIDS | |||
| E-β-Ocimene | 3016-19-1 | u.d. | u.d. |
| Limonene | 5989-27-5 | u.d. | u.d. |
| α-Phellandrene | 99-83-2 | 26.16 ± 0.52 | 20.07 ± 0.41 |
| β-Pinene | 18172-67-3 | u.d. | u.d. |
| (+)-α-Pinene | 7785-70-8 | u.d. | u.d. |
| β-Ionone | 79-77-6 | u.d. | u.d. |
| Myrcene | 123-35-3 | u.d. | u.d. |
| Nerolidol | 7212-44-4 | 27.23 ± 0.71 | 20.76 ± 0.55 |
| β-Caryophyllene | 87-44-5 | u.d. | u.d. |
| α-Humulene | 6753-98-6 | u.d. | u.d. |
| trans-β-Farnesene | 18794-84-8 | u.d. | u.d. |
| trans,trans-Farnesol | 106-28-5 | 25.21 ± 2.36 | 19.26 ± 1.78 |
| PUTATIVE SEX PHEROMONES | |||
| Hexyl butyrate | 2639-63-6 | u.d. | u.d. |
| Hexyl hexanoate | 6378-65-0 | u.d. | u.d. |
| Butyl butyrate | 109-21-7 | u.d. | u.d. |
| Ethyl butyrate | 105-54-4 | u.d. | u.d. |
| trans-2-hexenyl butyrate | 53398-83-7 | u.d. | u.d. |
| HOST PLANT SECONDARY METABOLITES | |||
| (−)-Catechin | 18829-704 | 19.26 ± 0.93 | 15.26 ± 0.70 |
| Rutin hydrate | 207671-50-9 | 9.89 ± 1.61 | 7.78 ± 1.23 |
| Quercetin | 117-39-5 | 3.36 ± 0.30 | 2.63 ± 0.23 |
| Gossypol | 303-45-7 | 4.45 ± 0.34 | 3.43 ± 0.32 |
U.d. means that the IC50 value exceeds 30 μM and thus that the binding affinities (Ki) of the candidate competitive ligand were not calculated in this study.