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. 2016 Jun 1;7:215. doi: 10.3389/fimmu.2016.00215

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Copyright © 2016 Chauhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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TLR3 on cell membrane with FcγRIIIa. Stimulated emission depletion (STED) confocal image of a human CD4⁺ T-cell activated with plate-bound anti-CD3 + ICs + sublytic C5b-9 post 48 h. FcγRIIIa recognized by binding of Alexa Fluor 488-labeled ICs (green) and TLR3 with monoclonal antibody-Alexa Fluor 594 (red). At the plasma membrane, both proteins colocalized in many spots. IC binding showed cytosolic FcγRIIIa (yellow oval) and on the plasma membrane (white arrow). Area amplified from merge shown in yellow rectangular.