Table 1.
Primers for cloning growth arrest-specific 2 into pAS4.1w.Ppuro-aOn plasmid.
| Official symbol | Accession | Sequence (from 5׳ to 3׳)a | Product size |
|---|---|---|---|
| GAS2 | NM_005256 | F: GCGATCGCGCTAGCATGTGCACTGCTCTGAGCCC | 964bp |
| R: GTTTAAACTCACTTAATTTCCTTCTTAGCCT |
Abbreviations: GAS2, growth arrest-specific 2; F, forward primer; R, reverse primer; pAS4.1w.Ppuro-aOn, all-in-one tetracycline-inducible plasmid.
a
Underline indicated the AsiSI restriction site at forward primer and the PmeI restriction site at reverse primer; start codon (ATG) and stop codon (TGA; reverse sequence: TCA) were in bold letters.